CD68 Rekombinanter Antikörper

CD68 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230504G10

Kat-Nr. : 83014-5-PBS

Synonyme

Scard1, gp110, CD68 antigen, 230504G10

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  WB analysis using 83014-5-RR (same clone as 83014-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83014-5-RR (CD68 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83014-5-RR (same clone as 83014-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 83014-5-RR (same clone as 83014-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 83014-5-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse spleen using 83014-5-RR (same clone as 83014-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

• 

  IF Staining of rat small intestine using 83014-5-RR (same clone as 83014-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat small intestine tissue using CD68 antibody (83014-5-RR, Clone: 230504G10 ) at dilution of 1:200 and Rhodamine (TRITC)-conjugated Goat Anti-Rabbit IgG(H+L) (SA00007-2), CD20 antibody (31909-1-AP, Magenta), EPCAM/CD326 antibody (32189-1-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83014-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83014-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83014-5-RR against Mouse CD68 were performed. The affinity constant is 8.77 nM.

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Geprüfte Anwendungen

Produktinformation

83014-5-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD68 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD68 fusion protein Eg0787
• Vollständiger Name: CD68 antigen
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 100-120 kDa
• GenBank-Zugangsnummer: NM_001291058.1
• Gene symbol: Cd68
• Gene ID (NCBI): 12514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.