CD68 Rekombinanter Antikörper

CD68 Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241810B3

Kat-Nr. : 84596-4-PBS

Synonyme

241810B3, CD68 molecule, GP110, Macrosialin, SCARD1

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  WB analysis using 84596-4-RR (same clone as 84596-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84596-4-RR (CD68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84596-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84596-4-RR (same clone as 84596-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84596-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84596-4-RR (same clone as 84596-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84596-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84596-4-RR (same clone as 84596-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84596-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 84596-4-RR (same clone as 84596-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84596-4-RR (CD68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84596-4-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 84596-4-RR (same clone as 84596-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD68 antibody (84596-4-RR, Clone: 241810B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84596-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84596-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD68 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD68 fusion protein Eg2061
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in the phagocytic activities of tissue macrophages.