CD68 Monoklonaler Antikörper

CD68 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

Y1/82A

Kat-Nr. : CL488-65187

Synonyme

CD68, CD68 molecule, GP110, Macrosialin, SCARD1, Y1/82A

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  FC experiment of human PBMCs using CL488-65187

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human CD68 (CL488-65187, Clone:Y1/82A), or CoraLite® Plus 488 Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were incubated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using CL488-65187

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 488 Anti-Human CD68 (CL488-65187, Clone:Y1/82A), or CoraLite® Plus 488 Mouse IgG2b Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were incubated with FC Receptor Block prior to staining.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65187 bindet in FC (Intra) CD68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lysosomaler Inhalt von Lungenmakrophagen
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.