CD68 Monoklonaler Antikörper

CD68 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Cardinal Red™ Fluorescent Dye

CloneNo.

KP1

Kat-Nr. : CR-65202

Synonyme

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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  FC experiment of human PBMCs using CR-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul Cardinal Red™ Anti-Human CD68 (CR-65202, Clone:KP1) (red) or unstained (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using CR-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul Cardinal Red™ Anti-Human CD68 (CR-65202, Clone:KP1) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CR-65202 bindet in FC (Intra) CD68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lysosomal fraction of human alveolar macrophages
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: Cardinal Red™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 592 nm / 611 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.