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CD68 Monoklonaler Antikörper

CD68 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

Y1/82A

Kat-Nr. : FITC-65187

Synonyme

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD68 (Y1/82A) (FITC-65187)

    FC experiment of human PBMCs using FITC-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD68 (Y1/82A) (FITC-65187)

    FC experiment of human PBMCs using FITC-65187

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65187, Clone:Y1/82A). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. Monocytes were gated.

Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65187 bindet in FC (Intra) CD68 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2b, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Lysosomaler Inhalt von Lungenmakrophagen
Vollständiger Name CD68 molecule
Berechnetes Molekulargewicht 37 kDa
GenBank-ZugangsnummerBC015557
Gene symbol CD68
Gene ID (NCBI) 968
Konjugation FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Form Liquid
Reinigungsmethode Mit Protein-A-Affinitätschromatographie gereinigt
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.