CD68 Monoklonaler Antikörper

CD68 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

PE Fluorescent Dye

Publikationen(4)

CloneNo.

Y1/82A

Kat-Nr. : PE-65187

Synonyme

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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  FC experiment of human PBMCs using PE-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65187

  1X10^6 human PBMCs were intracellularly stained with 5 ul PE Anti-Human CD68 (PE-65187, Clone:Y1/82A) or unstained. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC (Intra): humane PBMCs
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below
• FC: See 1 publications below

Produktinformation

PE-65187 bindet in IF, FC (Intra) CD68 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Lysosomaler Inhalt von Lungenmakrophagen
• Vollständiger Name: CD68 molecule
• Berechnetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC015557
• Gene symbol: CD68
• Gene ID (NCBI): 968
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Der gereinigte Antikörper ist mit R-Phycoerythrin (PE) unter optimalen Bedingungen konjugiert. Das Konjugat wird mit Größenausschluss-Chromatographie gereinigt.
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Publikationen

Species	Application	Title
	FC (Intra)	Cancer Sci; Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment; Authors - Anqi Feng
human	IF	Sci Adv; Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis; Authors - Shuwei Hu
human	FC	Int Immunopharmacol; OTUD4-mediated inhibition of YAP1 signaling pathway in ovarian cancer: Implications for macrophage polarization and recruitment; Authors - Mingyue Li
human	IF	Am J Cancer Res; FTO-mediated m6A modification of QPCT promotes tumorigenesis in lung adenocarcinoma by inducing macrophage chemotaxis and M2 polarization; Authors - Benkun Liu