CD69 Polyklonaler Antikörper

CD69 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 10803-1-AP

Synonyme

CLEC2C, CD69 molecule, BL-AC/P26, BL AC/P26, AIM

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  WB analysis using 10803-1-AP

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 10803-1-AP (CD69 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10803-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP (CD69 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 10803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD69 antibody (10803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human peripheral blood platelets
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 5 publications below
• FC: See 1 publications below

Produktinformation

10803-1-AP bindet in WB, IHC, IF-P, ELISA CD69 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD69 fusion protein Ag1094
• Vollständiger Name: CD69 molecule
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 28 kDa, 32 kDa
• GenBank-Zugangsnummer: BC007037
• Gene symbol: CD69
• Gene ID (NCBI): 969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.

Publikationen

Species	Application	Title
human	IHC	Cell Stem Cell; Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy; Authors - Meghan Logun
	IHC	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human	IF	iScience; Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis; Authors - Mick J M van Eijs
human	IF	Expert Rev Clin Immunol; Juxtaposition of IL-1β and IFN-γ expression and apoptosis of keratinocytes in adult-onset Still's disease.; Authors - Shijia Rao
	IF	Cell Rep; Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.; Authors - Huiwen Zheng
human	IHC	Oncoimmunology; Development and validation of an interferon signature predicting prognosis and treatment response for glioblastoma.; Authors - Chen Zhu