CD69 Rekombinanter Antikörper

CD69 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241552F1

Kat-Nr. : 98173-1-RR

Synonyme

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

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  FC experiment of human PBMCs using 98173-1-RR

  1x10^6 untreated or PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD4 (RPA-T4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98173-1-RR

  1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1) (red) or 0.25 ug Isotype Control, or 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98173-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98173-1-RR against Human CD69 were performed. The affinity constant is 0.257 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98173-1-RR bindet in FC CD69 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FusionProtein
• Vollständiger Name: CD69 molecule
• Berechnetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_001781.2
• Gene symbol: CD69
• Gene ID (NCBI): 969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).