CD69 Rekombinanter Antikörper

CD69 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241552G10

Kat-Nr. : 98173-2-PBS

Synonyme

Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule

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  FC experiment of human PBMCs using 98173-2-RR (same clone as 98173-2-PBS)

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone: 241552F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD4 (RPA-T4). Cells were not fixed. This data was developed using the same antibody clone with 98173-2-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98173-2-RR (same clone as 98173-2-PBS)

  1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone: 241552F1) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-2-RR, Clone:241552F1)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98173-2-PBS in a different storage buffer formulation.

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  FC experiment of Cnyo PBMCs using 98173-2-RR (same clone as 98173-2-PBS)

  1x10^6 Cnyo PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit RecAb (98173-2-RR, Clone:241552G10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98173-2-PBS in a different storage buffer formulation.

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  FC experiment of Cnyo PBMCs using 98173-2-RR (same clone as 98173-2-PBS)

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit RecAb (98173-2-RR, Clone: 241552G10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98173-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98173-2-PBS bindet in FC CD69 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD69 fusion protein Eg1791
• Vollständiger Name: CD69 molecule
• Berechnetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_001781.2
• Gene symbol: CD69
• Gene ID (NCBI): 969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).