CD69 Rekombinanter Antikörper

CD69 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

241552F1

Kat-Nr. : CL750-98173

Synonyme

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

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  FC experiment of human PBMCs using CL750-98173

  1x10^6 untreated or PHA treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD4 Rabbit Recombinant Antibody (CL488-98042, Clone: 240427E12) and 5 ul CoraLite® Plus 750 Anti-Human CD69 Rabbit Recombinant Antibody (CL750-98173, Clone: 241552F1). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-98173

  1x10^6 PHA treated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD69 Rabbit Recombinant Antibody (CL750-98173, Clone: 241552F1) (red) or CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD69 Rabbit Recombinant Antibody (CL750-98173, Clone:241552F1) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-98173 bindet in FC CD69 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD69 molecule
• Berechnetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: NM_001781.2
• Gene symbol: CD69
• Gene ID (NCBI): 969
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).