CD7 Monoklonaler Antikörper

CD7 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A4E6

Kat-Nr. : 60209-2-PBS

Synonyme

2A4E6, CD7 molecule, GP40, LEU-9, T cell antigen CD7

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  WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)

  Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse thymus using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 60209-2-Ig (same clone as 60209-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 60209-2-Ig(CD7 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60209-2-PBS bindet in WB, IHC, Indirect ELISA CD7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD7 fusion protein Ag1805
• Vollständiger Name: CD7 molecule
• Berechnetes Molekulargewicht: 240 aa, 25 kDa
• Beobachtetes Molekulargewicht: 34-36 kDa
• GenBank-Zugangsnummer: BC009293
• Gene symbol: CD7
• Gene ID (NCBI): 924
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).