CD7 Monoklonaler Antikörper

CD7 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

4H9

Kat-Nr. : 65203-1-Ig

Synonyme

4H9, CD7 molecule, GP40, LEU-9, T cell antigen CD7

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  FC experiment of human PBMCs using 65203-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD7 (65203-1-Ig, Clone:4H9) or Mouse IgG2a Isotype Control, and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500, then CL405 Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65203-1-Ig

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD7 (65203-1-Ig, Clone:4H9) or Mouse IgG2a Isotype Control, and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:500. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65203-1-Ig bindet in FC CD7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Zellen der T-Zell akuten lymphatischen Leukämie (T-Zell-ALL)
• Vollständiger Name: CD7 molecule
• Berechnetes Molekulargewicht: 240 aa, 25 kDa
• GenBank-Zugangsnummer: BC009293
• Gene symbol: CD7
• Gene ID (NCBI): 924
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).