CD7 Monoklonaler Antikörper

CD7 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4H9

Kat-Nr. : CL750-65203

Synonyme

4H9, CD7 molecule, GP40, LEU-9, T cell antigen CD7

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  FC experiment of human PBMCs using CL750-65203

  1X10^6 human PBMCs were surface co-stained with APC Anti-Human CD4 and 5 ul CoraLite® Plus 750 Anti-Human CD7 (CL750-65203, Clone:4H9) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL750-65203

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD7 (CL750-65203, Clone:4H9) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-65203 bindet in FC CD7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Zellen der T-Zell akuten lymphatischen Leukämie (T-Zell-ALL)
• Vollständiger Name: CD7 molecule
• Berechnetes Molekulargewicht: 240 aa, 25 kDa
• GenBank-Zugangsnummer: BC009293
• Gene symbol: CD7
• Gene ID (NCBI): 924
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD7, also known as Leu-9 or GP40, is a 40-kDa single-pass type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD7 is expressed on thymocytes, T cells, NK cells, and cells in the early stages of T, B, and myeloid cell differentiation (PMID: 10530432). It is one of the earliest antigens to appear on cells of the T-lymphocyte lineage (PMID: 3501369). CD7 plays a significant role in T-cell and T-cell/B-cell interactions during early lymphoid development. It is involved in the regulation of cell signaling and biology, including T-cell activation, proliferation, and the expression of interleukin-2 receptor alpha (IL-2Rα) (PMID: 11485208). CD7 is not only a marker for T-cells but also has functional implications in immune cell interactions and adhesion. It can provide co-stimulatory signals and is associated with CD3 and CD45, participating in T-cell activation (PMID: 7523512).