CD70 Rekombinanter Antikörper

CD70 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241553F2

Kat-Nr. : 98212-1-RR

Synonyme

241553F2, CD27 ligand, Cd27l, CD27-L, Cd27lg

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  FC experiment of Mouse splenic leucocytes using 98212-1-RR

  1x10^6 LPS treated Mouse splenic leucocytes were stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone:241553F2) or 1x10^6 LPS untreated Mouse splenic leucocytes were stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone:241553F2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Mouse splenic leucocytes using 98212-1-RR

  1x10^6 LPS treated mouse splenic leukocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 LPS treated Mouse splenic leucocytes were surface stained with 0.25 ug Anti-Mouse CD70 Rabbit Recombinant Antibody (98212-1-RR, Clone: 241553F2) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98212-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98212-1-RR against Mouse CD70 were performed. The affinity constant is 37.8 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: LPS treated mouse splenic leukocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98212-1-RR bindet in FC CD70 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD70 fusion protein Eg1294
• Vollständiger Name: CD70 antigen
• Berechnetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_011617.2
• Gene symbol: Cd70
• Gene ID (NCBI): 21948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD70, also known as tumor necrosis factor ligand superfamily member 7 (TNFSF7) or CD27 ligand (CD27L), is a transmembrane protein that plays a significant role in the immune system. It is the unique ligand for CD27, a member of the tumor necrosis factor receptor (TNFR) family, and is transiently upregulated upon stimulation of immune cells. CD70 is expressed on lymphocytes and dendritic cells, and its expression can be regulated by activation signals received via toll-like receptors (TLRs), CD40, and the antigen receptor MHC II. It provides complex and not completely understood signaling for B cell development. CD70 is also expressed on various solid tumors and hematolymphoid malignancies, where it may contribute to a poor prognosis. (PMID: 34991665; 26213107; 26098609)