CD71 Rekombinanter Antikörper

CD71 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

OKT9

Kat-Nr. : 65507-1-PBS

Synonyme

OKT9, p90, sTfR, T9, TFR

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  FC experiment of human PBMCs using 65507-1-PBS

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) or Rabbit IgG control Rabbit PolyAb and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65507-1-PBS

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (red) or Rabbit IgG control Rabbit PolyAb (blue); or 1x10^6 untreated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (black dashed). Cells were then stained with CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65507-1-PBS

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-PBS, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65507-1-RR (same clone as 65507-1-PBS)

  1x10^6 PHA treated human PBMCs were surface stained with 0.05 ug Anti-Human CD71 Rabbit Recombinant Antibody (65507-1-RR, Clone:OKT9) (left) and CoraLite®647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.05 ug Anti-Human CD71 Mouse Monoclonal Antibody (center and right) and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CL488 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65507-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65507-1-PBS bindet in FC CD71 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. Stable for one year after shipment.