CD71 Monoklonaler Antikörper

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• KD/KO Validated

CD71 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C11F11

Kat-Nr. : 66180-1-PBS

Synonyme

TFR1, TFR, T9, sTfR, p90

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  WB analysis of Raji using 66180-1-Ig (same clone as 66180-1-PBS)

  Untreated and PNGase F-treated lysates of Raji cells and HeLa cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  WB analysis using 66180-1-Ig (same clone as 66180-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

  WB result of CD71 antibody (66180-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  WB analysis of TF-1 using 66180-1-Ig (same clone as 66180-1-PBS)

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66180-1-Ig (same clone as 66180-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66180-1-Ig (same clone as 66180-1-PBS)

  IP result of anti-CD71 (IP:66180-1-Ig, 5ug; Detection:66180-1-Ig 1:1000) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 66180-1-Ig (same clone as 66180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66180-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66180-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA CD71 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD71 fusion protein Ag21612
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.