CD71 Rekombinanter Antikörper

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• KD/KO Validated

CD71 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2P14

Kat-Nr. : 81793-2-PBS

Synonyme

2P14, p90, sTfR, T9, TFR

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  WB analysis using 81793-2-RR (same clone as 81793-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 81793-2-RR (same clone as 81793-2-PBS)

  WB result of CD71 antibody (81793-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells. This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81793-2-RR (same clone as 81793-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 81793-2-RR (same clone as 81793-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

• 

  IF Staining of K-562 using 81793-2-RR (same clone as 81793-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 81793-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81793-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81793-2-RR against Human CD71 were performed. The affinity constant is 2.85 nM.

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Geprüfte Anwendungen

Produktinformation

81793-2-PBS bindet in WB, IF/ICC, Indirect ELISA CD71 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD71 fusion protein Ag0123
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.