CD71 Rekombinanter Antikörper

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• KD/KO Validated

CD71 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2P14

Kat-Nr. : 81793-2-RR

Synonyme

2P14, p90, sTfR, T9, TFR

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  WB analysis using 81793-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 81793-2-RR

  WB result of CD71 antibody (81793-2-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells.

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  WB analysis using 81793-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81793-2-RR (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 81793-2-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of K-562 using 81793-2-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-2-RR, Clone: 2P14 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 81793-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81793-2-RR against Human CD71 were performed. The affinity constant is 2.85 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, DU 145-Zellen, HeLa-Zellen, HT-1080.Zellen, K-562-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

81793-2-RR bindet in WB, IF/ICC, ELISA CD71 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD71 fusion protein Ag0123
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publikationen

Species	Application	Title
human	WB	Int Immunopharmacol; NLRP3 regulates ferroptosis via the JAK2/STAT3 pathway in asthma inflammation: Insights from in vivo and in vitro studies; Authors - Zhengze Zhang
mouse	IF	J Nanobiotechnology; Microglia-targeting nanosystems that cooperatively deliver Chinese herbal ingredients alleviate behavioral and cognitive deficits in Alzheimer's disease model mice; Authors - Peng Zhou