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CD71 Rekombinanter Antikörper

CD71 Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250738C5

Kat-Nr. : 81793-3-PBS

Synonyme

p90, sTfR, T9, TFR, TFR1

Filter:
  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Recombinant monoclonal antibody (81793-3-RR)

    IHC staining of human placenta using 81793-3-RR (same clone as 81793-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81793-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CD71 Recombinant monoclonal antibody (81793-3-RR)

    IHC staining of human placenta using 81793-3-RR (same clone as 81793-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81793-3-RR (CD71 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81793-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CD71 Recombinant monoclonal antibody (81793-3-RR)

    IF Staining of K-562 using 81793-3-RR (same clone as 81793-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (81793-3-RR, Clone: 250738C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81793-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81793-3-RR

    Affinity and Kinetic Characterization of 81793-3-RR

    Biolayer interferometry (BLl) kinetic assays of 81793-3-RR against Human CD71 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

81793-3-PBS bindet in IHC, IF/ICC, Indirect ELISA CD71 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD71 fusion protein Eg0339
Vollständiger Name transferrin receptor (p90, CD71)
Berechnetes Molekulargewicht85 kDa
Gene symbol CD71
Gene ID (NCBI) 7037
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.