CD71 Monoklonaler Antikörper

CD71 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(1)

CloneNo.

OKT9

Kat-Nr. : FITC-65235

Synonyme

OKT9, p90, sTfR, T9, TFR

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  FC experiment of human PBMCs using FITC-65235

  1x10^6 PHA-treated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 (OKT9) (FITC-65235, Clone: OKT9) (red) or FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124, Clone: MOPC-21) (blue). Untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 (OKT9) (FITC-65235, Clone: OKT9) (black, dashed). Cells were not fixed.

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  FC experiment of Human PBMC using FITC-65235

  1X10^6 PHA-activated human PBMC were surface stained with 5 ul FITC Plus Anti-Human CD71 (FITC-65235, Clone: OKT9) or Mouse IgG1 Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

FITC-65235 bindet in IF, FC CD71 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publikationen

Species	Application	Title
human	IF	Cell Chem Biol; Anti-tumor immunotherapy using engineered bacterial outer membrane vesicles fused to lysosome-targeting chimeras mediated by transferrin receptor; Authors - Ling-Yan Su