CD71 Rekombinanter Antikörper

CD71 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

OKT9

Kat-Nr. : FITC-65507

Synonyme

OKT9, p90, sTfR, T9, TFR

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  FC experiment of human PBMCs using FITC-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD71 Rabbit Recombinant Antibody (FITC-65507, Clone:OKT9) (right) or FITC Plus-conjugated Rabbit IgG control PolyAb (FITC-30000) (left). 1X10^6 unactivated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 Mouse Monoclonal Antibody (center). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 Rabbit Recombinant Antibody (FITC-65507, Clone:OKT9) (red) or FITC Plus-conjugated Rabbit IgG control PolyAb (FITC-30000) (blue). 1X10^6 unactivated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD71 Mouse Monoclonal Antibody (black dashed). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65507

  1X10^6 PHA-activated (3 day) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD71 Rabbit Recombinant Antibody (FITC-65507, Clone:OKT9) (left) or 5 ul FITC Plus Anti-Human CD71 Mouse Monoclonal Antibody (FITC-65235, Clone:OKT9) (right). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated (3 day) human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65507 bindet in FC CD71 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human T-cell acute lymphoblastic leukemia cells
• Vollständiger Name: transferrin receptor (p90, CD71)
• Berechnetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC001188
• Gene symbol: CD71
• Gene ID (NCBI): 7037
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.