CD72 Rekombinanter Antikörper

CD72 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241301B3

Kat-Nr. : 84270-5-RR

Synonyme

241301B3, B-cell differentiation antigen CD72, CD72 molecule, CD72b, Lyb 2

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  WB analysis of Raji using 84270-5-RR

  Raji cells were subjected to SDS PAGE followed by western blot with 84270-5-RR (CD72 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of Ramos using 84270-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD72 antibody (84270-5-RR, Clone: 241301B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of Raji using 84270-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD72 antibody (84270-5-RR, Clone: 241301B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84270-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84270-5-RR against Human CD72 were performed. The affinity constant is 59.3 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: Ramos-Zellen, Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84270-5-RR bindet in WB, IF/ICC, ELISA CD72 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD72 molecule
• Berechnetes Molekulargewicht: 359 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC030227
• Gene symbol: CD72
• Gene ID (NCBI): 971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).