CD72 Rekombinanter Antikörper

CD72 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241300C1

Kat-Nr. : 98090-1-RR

Synonyme

241300C1, B-cell differentiation antigen CD72, CD72 molecule, CD72b, Lyb 2

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  FC experiment of human PBMCs using 98090-1-RR

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD72 Rabbit RecAb (98090-1-RR, Clone: 241300C1) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 750 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL750-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using 98090-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD72 Rabbit RecAb (98090-1-RR, Clone:241300C1) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

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  Affinity and Kinetic Characterization of 98090-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98090-1-RR against Human CD72 were performed. The affinity constant is 10.7 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98090-1-RR bindet in FC CD72 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD72 molecule
• Berechnetes Molekulargewicht: 359 aa, 40 kDa
• GenBank-Zugangsnummer: BC030227
• Gene symbol: CD72
• Gene ID (NCBI): 971
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).