CD73 Monoklonaler Antikörper

CD73 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

AD2

Kat-Nr. : 65162-1-Ig

Synonyme

NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73)

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  FC experiment of human PBMCs using 65162-1-Ig

  1X10^6 human PBMCs were surface co-stained with CL405 Anti-Human CD3 and 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65162-1-Ig

  1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD73 (65162-1-Ig, Clone:AD2) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

65162-1-Ig bindet in FC CD73 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Pre-B leukemia cell line
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 29 kDa, 63 kDa
• GenBank-Zugangsnummer: BC015940
• Gene symbol: CD73
• Gene ID (NCBI): 4907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).

Publikationen

Species	Application	Title
human	FC	Exp Ther Med; Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts.; Authors - Junjie Liu
	FC	Dis Markers; Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis; Authors - Baihe Han