CD73 Rekombinanter Antikörper

CD73 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

251250H3

Kat-Nr. : 98566-1-PBS

Synonyme

NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

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  FC experiment of human PBMCs using 98566-1-RR (same clone as 98566-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 Rabbit RecAb (98566-1-RR, Clone:251250H3) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3). Cells were not fixed. This data was developed using the same antibody clone with 98566-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98566-1-RR (same clone as 98566-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD73 Rabbit RecAb (98566-1-RR, Clone: 251250H3) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98566-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98566-1-PBS bindet in FC CD73 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD73 fusion protein Eg2974
• Vollständiger Name: 5'-nucleotidase, ecto (CD73)
• Berechnetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: NM_002526.3
• Gene symbol: CD73
• Gene ID (NCBI): 4907
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).