CD74 Monoklonaler Antikörper
CD74 Monoklonal Antikörper für WB, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2a
Getestete Reaktivität
human
Anwendung
WB, IF/ICC, ELISA
Konjugation
Unkonjugiert
CloneNo.
2D8A1
Kat-Nr. : 66390-1-Ig
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Raji-Zellen, Daudi-Zellen, Ramos-Zellen |
Erfolgreiche Detektion in IF/ICC | Raji-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 3 publications below |
IF | See 4 publications below |
Produktinformation
66390-1-Ig bindet in WB, IF/ICC, ELISA CD74 und zeigt Reaktivität mit human
Getestete Reaktivität | human |
In Publikationen genannte Reaktivität | human |
Wirt / Isotyp | Maus / IgG2a |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | CD74 fusion protein Ag25503 |
Vollständiger Name | CD74 molecule, major histocompatibility complex, class II invariant chain |
Berechnetes Molekulargewicht | 34 kDa |
Beobachtetes Molekulargewicht | 33 kDa |
GenBank-Zugangsnummer | BC018726 |
Gene symbol | CD74 |
Gene ID (NCBI) | 972 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165;PMID: 36712241).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for CD74 antibody 66390-1-Ig | Protokoll herunterladen |
IF protocol for CD74 antibody 66390-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Stem Cell Res Ther DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis | ||
Int Immunol Helicobacter urease suppresses cytotoxic CD8 + T cell responses through activating Myh9-dependent induction of PD-L1. | ||
Neural Regen Res Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect | ||
Nat Commun Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment | ||
Am J Physiol Cell Physiol WISP1 induces the expression of macrophage migration inhibitory factor (MIF) in human lung fibroblasts through Src kinases and EGFR-activated signaling pathways | ||
bioRxiv Proteomic analysis reveals the dominant effect of ipomoeassin F on the synthesis of membrane and secretory proteins in triple-negative breast cancer cells |