CD74 Monoklonaler Antikörper

CD74 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

2D8A1

Kat-Nr. : 66390-1-Ig

Synonyme

Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide



Geprüfte Anwendungen

Erfolgreiche Detektion in WBRaji-Zellen, Daudi-Zellen, Ramos-Zellen
Erfolgreiche Detektion in IF/ICCRaji-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 3 publications below
IFSee 4 publications below

Produktinformation

66390-1-Ig bindet in WB, IF/ICC, ELISA CD74 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen CD74 fusion protein Ag25503
Vollständiger Name CD74 molecule, major histocompatibility complex, class II invariant chain
Berechnetes Molekulargewicht 34 kDa
Beobachtetes Molekulargewicht 33 kDa
GenBank-ZugangsnummerBC018726
Gene symbol CD74
Gene ID (NCBI) 972
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165;PMID: 36712241).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CD74 antibody 66390-1-IgProtokoll herunterladen
IF protocol for CD74 antibody 66390-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Stem Cell Res Ther

DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis

Authors - S Y Wang
WB

Int Immunol

Helicobacter urease suppresses cytotoxic CD8 + T cell responses through activating Myh9-dependent induction of PD-L1.

Authors - Jian Wu
IF

Neural Regen Res

Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect

Authors - Piniel Alphayo Kambey
humanIF

Nat Commun

Single-cell multi-omics analysis of human testicular germ cell tumor reveals its molecular features and microenvironment

Authors - Xiaojian Lu
humanWB

Am J Physiol Cell Physiol

WISP1 induces the expression of macrophage migration inhibitory factor (MIF) in human lung fibroblasts through Src kinases and EGFR-activated signaling pathways

Authors - Maria-Elpida Christopoulou
humanWB

bioRxiv

Proteomic analysis reveals the dominant effect of ipomoeassin F on the synthesis of membrane and secretory proteins in triple-negative breast cancer cells

Authors - Brihget Sicairos