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CD74 Monoklonaler Antikörper

CD74 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2D8A1

Kat-Nr. : 66390-1-PBS

Synonyme

Ia antigen-associated invariant chain, HLA-DR antigens-associated invariant chain, HLADG, DHLAG, Class-II-associated invariant chain peptide

Filter:
  • Western Blot (WB) analysis of various lysates using CD74 Monoclonal antibody (66390-1-Ig)

    WB analysis using 66390-1-Ig (same clone as 66390-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using CD74 Monoclonal antibody (66390-1-Ig)

    WB analysis of Ramos using 66390-1-Ig (same clone as 66390-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Daudi cells using CD74 Monoclonal antibody (66390-1-Ig)

    WB analysis of Daudi using 66390-1-Ig (same clone as 66390-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD74 Monoclonal antibody (66390-1-Ig)

    IF Staining of Raji using 66390-1-Ig (same clone as 66390-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD74 antibody (66390-1-Ig, Clone: 2D8A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66390-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66390-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD74 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen CD74 fusion protein Ag25503
Vollständiger Name CD74 molecule, major histocompatibility complex, class II invariant chain
Berechnetes Molekulargewicht 34 kDa
Beobachtetes Molekulargewicht 33 kDa
GenBank-ZugangsnummerBC018726
Gene symbol CD74
Gene ID (NCBI) 972
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165;PMID: 36712241).