CD79a Polyklonaler Antikörper

CD79a Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31887-1-AP

Synonyme

B-cell antigen receptor complex-associated protein alpha chain, Mb-1

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  WB analysis using 31887-1-AP

  Mouse spleen tissue was subjected to SDS PAGE, followed by western blot with 31887-1-AP (CD79a antibody) at a dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat spleen using 31887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD79a antibody (31887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Rattenmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31887-1-AP bindet in WB, IHC, IF-P, ELISA CD79a und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD79a fusion protein Eg1388
• Vollständiger Name: CD79A antigen (immunoglobulin-associated alpha)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_007655.3
• Gene symbol: Cd79a
• Gene ID (NCBI): 12518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.