CD79a Rekombinanter Antikörper

CD79a Rekombinant Antikörper für IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1I17

Kat-Nr. : 82747-2-RR

Synonyme

1I17, B-cell antigen receptor complex-associated protein alpha chain, MB 1, MB 1 membrane glycoprotein, MB1

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  IF Staining of mouse spleen using 82747-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD79a antibody (82747-2-RR, Clone: 1I17 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 82747-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD79A antibody (82747-2-RR, Clone: 1I17 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82747-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82747-2-RR against Human CD79A were performed. The affinity constant is 8.32 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82747-2-RR bindet in IF/ICC, IF-P, ELISA CD79a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD79a fusion protein Ag17924
• Vollständiger Name: CD79a molecule, immunoglobulin-associated alpha
• Berechnetes Molekulargewicht: 226 aa, 25 kDa
• GenBank-Zugangsnummer: BC113733
• Gene symbol: CD79A
• Gene ID (NCBI): 973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.