CD79b Polyklonaler Antikörper

CD79b Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21063-1-AP

Synonyme

Ig-beta, IGB, Ig beta, B-cell-specific glycoprotein B29, B-cell antigen receptor complex-associated protein beta chain

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  WB analysis of Raji using 21063-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21063-1-AP (CD79B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79b antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:600 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21063-1-AP (CD79B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21063-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21063-1-AP (CD79B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, Maus-Kolongewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 4 publications below

Produktinformation

21063-1-AP bindet in WB, IHC, IF, ELISA CD79b und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD79b fusion protein Ag15086
• Vollständiger Name: CD79b molecule, immunoglobulin-associated beta
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC032651
• Gene symbol: CD79B
• Gene ID (NCBI): 974
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).

Publikationen

Species	Application	Title
human	IF	Front Immunol; CD20+CD22+ADAM28+ B Cells in Tertiary Lymphoid Structures Promote Immunotherapy Response.; Authors - Zhenghao Wu
mouse	IF	Cell Discov; Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure; Authors - Jie Yin
human	IF	J Nanobiotechnology; Targeted degradation of oncogenic BCR-ABL by silencing the gene of NEDD8 E3 ligase RAPSYN; Authors - Yanzi Sun
human	IF	Int Endod J; FoxO1 mediates odontoblast differentiation of hDPSCs via B cell-derived ANGPTL1 in dental caries: A laboratory investigation; Authors - Peimeng Zhan