CD79a Rekombinanter Antikörper

CD79a Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

HM57

Kat-Nr. : 65610-1-PBS

Synonyme

MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1, HM57

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  FC experiment of human PBMCs using 65610-1-MR (same clone as 65610-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a (HM57) Mouse IgG2a RecAb (65610-1-MR, Clone:HM57) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated. This data was developed using the same antibody clone with 65610-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65610-1-MR (same clone as 65610-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CD79a (HM57) Mouse IgG2a RecAb (65610-1-MR, Clone:HM57)(red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4)(blue) and PE-conjugated Goat Anti-Mouse IgG2a. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated. This data was developed using the same antibody clone with 65610-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65610-1-PBS bindet in FC (Intra) CD79a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of human CD79a
• Vollständiger Name: CD79a molecule, immunoglobulin-associated alpha
• GenBank-Zugangsnummer: BC113731
• Gene symbol: CD79A
• Gene ID (NCBI): 973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79a is expressed in B cell and localizes in the cell membrane. CD79a is required in cooperation with CD79b for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79a is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.