CD79a Rekombinanter Antikörper

CD79a Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241299F1

Kat-Nr. : 84236-4-PBS

Synonyme

MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1, B-cell antigen receptor complex-associated protein alpha chain

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  WB analysis using 84236-4-RR (same clone as 84236-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84236-4-RR (CD79a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84236-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84236-4-RR (same clone as 84236-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84236-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84236-4-RR (same clone as 84236-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84236-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84236-4-RR (same clone as 84236-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84236-4-RR (CD79a antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84236-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84236-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84236-4-RR against Human CD79a were performed. The affinity constant is 20.8 nM.

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Geprüfte Anwendungen

Produktinformation

84236-4-PBS bindet in WB, IHC, Indirect ELISA CD79a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD79a molecule, immunoglobulin-associated alpha
• Berechnetes Molekulargewicht: 226 aa, 25 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC113733
• Gene symbol: CD79A
• Gene ID (NCBI): 973
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.