CD79b Rekombinanter Antikörper

CD79b Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241273B7

Kat-Nr. : 84234-5-RR

Synonyme

241273B7, B29, B-cell antigen receptor complex-associated protein beta chain, B-cell-specific glycoprotein B29, Ig beta

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  WB analysis using 84234-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84234-5-RR (CD79b antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 84234-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84234-5-RR (CD79b antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 84234-5-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84234-5-RR (CD79b antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Raji using 84234-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD79b antibody (84234-5-RR, Clone: 241273B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84234-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84234-5-RR against Human CD79b were performed. The affinity constant is 3.23 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84234-5-RR bindet in WB, IHC, IF/ICC, ELISA CD79b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD79b molecule, immunoglobulin-associated beta
• Berechnetes Molekulargewicht: 229 aa, 26 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC032651
• Gene symbol: CD79B
• Gene ID (NCBI): 974
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD79 molecule encompasses two transmembrane proteins, CD79a and CD79b, which form a disulfide-linked heterodimer and are members of the immunoglobulin (Ig) gene superfamily (PMID: 2033258; 1375264; 2304550). CD79b molecule (also known as B29, IGB, AGM6, and Igbeta) is expressed almost exclusively on B cells and B-cell neoplasms, influencing antigen internalization and increasing antigen presentation efficiency (PMID: 7552998). CD79b mutation could be a key biomarker for DLBCL disease progression (PMID: 36182550).