CD8 Rekombinanter Antikörper

CD8 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

SK1

Kat-Nr. : 65630-1-PBS

Synonyme

CD8A, CD8a molecule, Leu2, MAL, p32

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  FC experiment of human PBMCs using 65630-1-MR (same clone as 65630-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (SK1) Mouse IgG2a Recombinant Antibody (65630-1-MR, Clone: SK1) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with APC Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65630-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65630-1-MR (same clone as 65630-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD8 (SK1) Mouse IgG2a Recombinant Antibody (65630-1-MR, Clone: SK1) (red) or 0.25 ug Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65630-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Cnyo PBMCs using 65630-1-MR (same clone as 65630-1-PBS)

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD8 (SK1) Mouse IgG2a RecAb (65630-1-MR, Clone:SK1) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65630-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Cnyo PBMCs using 65630-1-MR (same clone as 65630-1-PBS)

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD8 (SK1) Mouse IgG2a RecAb (65630-1-MR, Clone:SK1) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65630-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65630-1-PBS bindet in FC CD8 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human peripheral blood T lymphocytes
• Vollständiger Name: CD8a molecule
• Berechnetes Molekulargewicht: 235 aa, 26 kDa
• GenBank-Zugangsnummer: BC025715
• Gene symbol: CD8A
• Gene ID (NCBI): 925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).