CD8 Monoklonaler Antikörper

CD8 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1G2B10

Kat-Nr. : CL594-66868

Synonyme

CD8, CD8A, CD8a molecule, Leu2, MAL, p32

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10 ) at dilution of 1:400. DAPI (blue).

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  IF Staining of human colon cancer using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Magenta) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

• 

  IF Staining of human colon cancer using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488 pan-keratin antibody (CL488-26411) at dilution of 1:200, CoraLite® Plus 647 CD3 antibody (CL647-60181, Clone: 3F3A1, Cyan) at dilution of 1:100, CoraLite®594 CD8 antibody (CL594-66868, Clone: 1G2B10, Red ) at dilution of 1:400. DAPI (Blue).

• 

  IF Staining of human tonsillitis using CL594-66868

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL594-66868 (CD8 antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Appendizitis-Gewebe, humanes Kolonkarzinomgewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66868 bindet in IF CD8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD8 fusion protein Ag11111
• Vollständiger Name: CD8a molecule
• Berechnetes Molekulargewicht: 235 aa, 26 kDa
• GenBank-Zugangsnummer: BC025715
• Gene symbol: CD8A
• Gene ID (NCBI): 925
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.