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CD8 Monoklonaler Antikörper

CD8 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

SK1

Kat-Nr. : CL647-65146

Synonyme

CD8A, CD8a molecule, Leu2, MAL, p32

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD8 (SK1) (CL647-65146)

    FC experiment of human PBMCs using CL647-65146

    1X10^6 human PBMCs were surface co-stained with CoraLite® Plus 405 Anti-Human CD3 and 5 ul CoraLite® Plus 647 Anti-Human CD8 (CL647-65146, Clone:SK1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 647 Anti-Human CD8 (SK1) (CL647-65146)

    FC experiment of human PBMCs using CL647-65146

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD8 (CL647-65146, Clone:SK1) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

Geprüfte Anwendungen

Erfolgreiche Detektion in FChumane PBMCs

Empfohlene Verdünnung

AnwendungVerdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65146 bindet in FC CD8 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Human peripheral blood T lymphocytes
Vollständiger Name CD8a molecule
Berechnetes Molekulargewicht 235 aa, 26 kDa
GenBank-ZugangsnummerBC025715
Gene symbol CD8A
Gene ID (NCBI) 925
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS with 0.09% sodium azide and 0.5% BSA
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
FC protocol for CL Plus 647 CD8 antibody CL647-65146Download protocol
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sunil (Verified Customer) (07-24-2023)

worked as expected and as per the datasheet, nice to use in a flow panel

  • Applications: Flow Cytometry