CD8 Rekombinanter Antikörper

CD8 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

CoraLite®700 Fluorescent Dye

CloneNo.

SK1

Kat-Nr. : CL700-65630

Synonyme

CD8A, CD8a molecule, Leu2, MAL, p32

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  FC experiment of human PBMCs using CL700-65630

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD3, and 5 ul CoraLite®700 Anti-Human CD8 (SK1) Mouse IgG2a RecAb (CL700-65630, Clone: SK1) or unstained. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL700-65630

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite®700 Anti-Human CD8 (SK1) Mouse IgG2a RecAb (CL700-65630, Clone:SK1) (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL700-65630 bindet in FC CD8 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Human peripheral blood T lymphocytes
• Vollständiger Name: CD8a molecule
• Berechnetes Molekulargewicht: 235 aa, 26 kDa
• GenBank-Zugangsnummer: BC025715
• Gene symbol: CD8A
• Gene ID (NCBI): 925
• Konjugation: CoraLite®700 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 702 nm / 723 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).