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CD80 Rekombinanter Antikörper

CD80 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250725H4

Kat-Nr. : 86532-1-PBS

Synonyme

B7 1, B7.1, B7-1, Cd80 molecule

Filter:
  • Western Blot (WB) analysis of NR8383 cells using CD80 Recombinant antibody (86532-1-RR)

    WB analysis of NR8383 using 86532-1-RR (same clone as 86532-1-PBS)

    NR8383 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (CD80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD80 Recombinant antibody (86532-1-RR)

    WB analysis using 86532-1-RR (same clone as 86532-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD80 Recombinant antibody (86532-1-RR)

    WB analysis using 86532-1-RR (same clone as 86532-1-PBS)

    C2C12 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CD80 Recombinant antibody (86532-1-RR)

    IF Staining of C2C12 using 86532-1-RR (same clone as 86532-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CD80 antibody (86532-1-RR, Clone: 250725H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86532-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD80 und zeigt Reaktivität mit Maus, Ratten

Getestete Reaktivität Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD80 fusion protein Eg3841
Vollständiger Name Cd80 molecule
Berechnetes Molekulargewicht33 kDa
Beobachtetes Molekulargewicht60-70 kDa
Gene symbol CD80
Gene ID (NCBI) 25408
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)