CD80 Rekombinanter Antikörper

CD80 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250725H4

Kat-Nr. : 86532-1-RR

Synonyme

B7 1, B7.1, B7-1, Cd80 molecule

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  WB analysis of NR8383 using 86532-1-RR

  NR8383 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (CD80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86532-1-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86532-1-RR

  C2C12 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of C2C12 using 86532-1-RR

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CD80 antibody (86532-1-RR, Clone: 250725H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NR8383 cells, C2C12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86532-1-RR bindet in WB, IF/ICC, ELISA CD80 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD80 fusion protein Eg3841
• Vollständiger Name: Cd80 molecule
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• Gene symbol: CD80
• Gene ID (NCBI): 25408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)