CD80 Rekombinanter Antikörper

CD80 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

241371E12

Kat-Nr. : CL594-98158

Synonyme

241371E12, B7 1, B7.1, Cd28l, CD80 antigen

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  FC experiment of mouse peritoneal macrophages using CL594-98158

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD80 Rabbit RecAb (CL594-98158, Clone: 241371E12) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using CL594-98158

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD80 Rabbit RecAb (CL594-98158, Clone: 241371E12) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98158 bindet in FC CD80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD80 fusion protein Eg0840
• Vollständiger Name: CD80 antigen
• Berechnetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_009855.2
• Gene symbol: CD80
• Gene ID (NCBI): 12519
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)