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CD80 Polyklonaler Antikörper

CD80 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14292-1-PBS

Synonyme

BB1, B7-1, B7 1, B7, Activation B7-1 antigen

Filter:
  • Western Blot (WB) analysis of various lysates using CD80 Polyclonal antibody (14292-1-AP)

    WB analysis using 14292-1-AP (same clone as 14292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using CD80 Polyclonal antibody (14292-1-AP)

    WB analysis of human peripheral blood leukocyte using 14292-1-AP (same clone as 14292-1-PBS)

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14292-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using CD80 Polyclonal antibody (14292-1-AP)

    WB analysis of RAW 264.7 using 14292-1-AP (same clone as 14292-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14292-1-AP (CD80/B7-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14292-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14292-1-PBS bindet in WB, Indirect ELISA CD80 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD80 fusion protein Ag5615
Vollständiger Name CD80 molecule
Berechnetes Molekulargewicht 33 kDa
Beobachtetes Molekulargewicht 60 kDa
GenBank-ZugangsnummerBC042665
Gene symbol CD80
Gene ID (NCBI) 941
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)