CD80 (B7-1) Monoklonaler Antikörper

CD80 (B7-1) Monoklonal Antikörper für FC

Wirt / Isotyp

Armenian Hamster / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

16-10A1

Kat-Nr. : 65076-1-Ig

Synonyme

B7 1, B7.1, Cd28l, Cd80, CD80 (B7-1), CD80 antigen, Ly 53, Ly53, MIC17, TS/A 1

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Galerie der Validierungsdaten

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  FC experiment of BALB/C mouse peritoneal macrophages using 65076-1-Ig

  1X10^6 BALB/c mouse peritoneal macrophages were surface stained with 0.5 ug Anti-Mouse CD80 (B7-1) (65076-1-Ig, Clone:16-10A1) and FITC anti-Armenian Hamster IgG Antibody at dilution 1:100 or unstained. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 1 publications below

Produktinformation

65076-1-Ig bindet in FC CD80 (B7-1) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Armenian Hamster / IgG
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHO-Zelllinie transfiziert mit Maus-B7 (CD80)
• Vollständiger Name: CD80 antigen
• GenBank-Zugangsnummer: BC131959
• Gene symbol: Cd80
• Gene ID (NCBI): 12519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid, pH 7,2.
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)

Publikationen

Species	Application	Title
mouse	FC	Biomolecules; Glucose Transporter Glut1-Dependent Metabolic Reprogramming Regulates Lipopolysaccharide-Induced Inflammation in RAW264.7 Macrophages; Authors - Alex Cornwell