CD80 Rekombinanter Antikörper

CD80 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

3H5

Kat-Nr. : 65580-1-PBS

Synonyme

Cd80 molecule, B7-1, B7.1, B7 1, 3H5

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  FC experiment of rat splenocytes using 65580-1-MR (same clone as 65580-1-PBS)

  1x10^6 unstimulated or LPS-stimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5) and PE-Conjugated Goat Anti-Mouse IgG. Cells were then stained with CoraLite® Plus 647 Anti-Rat CD45RA. Cells were not fixed. This data was developed using the same antibody clone with 65580-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65580-1-MR (same clone as 65580-1-PBS)

  1x10^6 LPS-stimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and PE-Conjugated Goat Anti-Mouse IgG. 1x10^6 unstimulated rat splenocytes were surface stained with 0.25 ug Anti-Rat CD80/B7-1 Mouse IgG2a Recombinant Antibody (65580-1-MR, Clone: 3H5)(black, dashed) and PE-Conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65580-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65580-1-PBS bindet in FC CD80 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HTLV-1-transformed Lewis-S1 rat T cell line
• Vollständiger Name: Cd80 molecule
• Berechnetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: XM_017597885
• Gene symbol: CD80
• Gene ID (NCBI): 25408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)