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CD80 Rekombinanter Antikörper

CD80 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241371E10

Kat-Nr. : 84137-5-PBS

Synonyme

Ly 53, CD80 antigen, Cd28l, B7.1, B7 1

Filter:
  • Western Blot (WB) analysis of mouse spleen tissue using CD80 Recombinant antibody (84137-5-RR)

    WB analysis of mouse spleen using 84137-5-RR (same clone as 84137-5-PBS)

    mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84137-5-RR (CD80/B7-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84137-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84137-5-RR

    Affinity and Kinetic Characterization of 84137-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84137-5-RR against Mouse CD80/B7-1 were performed. The affinity constant is 0.204 nM.

Geprüfte Anwendungen

Produktinformation

84137-5-PBS bindet in WB, Indirect ELISA CD80 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name CD80 antigen
Berechnetes Molekulargewicht35 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerNM_009855.2
Gene symbol CD80
Gene ID (NCBI) 12519
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)