CD80 Rekombinanter Antikörper

CD80 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241371E12

Kat-Nr. : 98158-1-PBS

Synonyme

Ly 53, CD80 antigen, Cd28l, B7.1, B7 1

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  FC experiment of mouse peritoneal macrophages using 98158-1-RR (same clone as 98158-1-PBS)

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98158-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse peritoneal macrophages using 98158-1-RR (same clone as 98158-1-PBS)

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98158-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98158-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98158-1-RR against Mouse CD80/B7-1 were performed. The affinity constant is 1.05 nM.

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Geprüfte Anwendungen

Produktinformation

98158-1-PBS bindet in FC CD80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD80 antigen
• Berechnetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_009855.2
• Gene symbol: CD80
• Gene ID (NCBI): 12519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)