CD80 Rekombinanter Antikörper

CD80 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241371E12

Kat-Nr. : 98158-1-RR

Synonyme

Ly 53, CD80 antigen, Cd28l, B7.1, B7 1

---

• 

  FC experiment of mouse peritoneal macrophages using 98158-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using 98158-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD80/B7-1 Rabbit Recombinant Antibody (98158-1-RR, Clone: 241371E12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98158-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98158-1-RR against Mouse CD80/B7-1 were performed. The affinity constant is 1.05 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98158-1-RR bindet in FC CD80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD80 antigen
• Berechnetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: NM_009855.2
• Gene symbol: CD80
• Gene ID (NCBI): 12519
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)