CD81 Monoklonaler Antikörper

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• KD/KO Validated

CD81 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(176)

CloneNo.

1G2C6

Rezensionen (1)

Kat-Nr. : 66866-1-Ig

Synonyme

1G2C6, 26 kDa cell surface protein TAPA-1, CD81 antigen, CD81 molecule, S5.7

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• 

  WB analysis using 66866-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 66866-1-Ig

  WB result of CD81 antibody (66866-1-Ig; 1:3000; room temperature for 1.5 hours) with wild-type and CD81 knockout HCT 116 cells.

• 

  WB analysis using 66866-1-Ig

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66866-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66866-1-Ig

  LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 66866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66866-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66866-1-Ig (CD81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66866-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66866-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD81 antibody (66866-1-Ig, Clone: 1G2C6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HCT 116-Zellen, HEK-293-Zellen, Jurkat-Zellen, mit LPS behandelte THP-1-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 183 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Produktinformation

66866-1-Ig bindet in WB, IHC, IF-P, ELISA CD81 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD81 fusion protein Ag27298
• Vollständiger Name: CD81 molecule
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC002978
• Gene symbol: CD81
• Gene ID (NCBI): 975
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).

Publikationen

Species	Application	Title
human	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang
human	WB	J Am Chem Soc; Efficient Metabolomics Profiling from Plasma Extracellular Vesicles Enables Accurate Diagnosis of Early Gastric Cancer; Authors - Fanqin Bu
human	WB	J Extracell Vesicles; Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation.; Authors - Bowen Liu
	WB	Sci Adv; Avian ANP32A incorporated in avian influenza A virions promotes interspecies transmission by priming early viral replication in mammals; Authors - Lei Na
human	WB	J Exp Clin Cancer Res; Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization; Authors - Yuanshan Yao
human	WB,IF	J Exp Clin Cancer Res; Chemotherapy-elicited extracellular vesicle CXCL1 from dying cells promotes triple-negative breast cancer metastasis by activating TAM/PD-L1 signaling; Authors - Shengqi Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaogang (Verified Customer) (10-21-2021)

I recently bought this CD81 antibody to detect CD81 from EVs purified from human and mouse cell lines. The antibody works well to detect CD81 by immunoblot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: RAW264.7 and differentiated THP-1 cells

CD81 Antibody Western Blot validation (1:1000 dilution) in RAW264.7 and differentiated THP-1 cells (Cat no:66866-1-Ig)