CD81 Monoklonaler Antikörper

CD81 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G2C6

Kat-Nr. : CL488-66866

Synonyme

CD81, CD81 antigen, CD81 molecule, Exosome marker, S5.7, TAPA1, Tetraspanin 28, Tspan 28, TSPAN28

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Galerie der Validierungsdaten

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  IF Staining of human tonsillitis using CL488-66866

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated CD81 antibody (CL488-66866, Clone: 1G2C6 ) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL488-66866

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite® Plus 488-conjugated CD81 antibody (CL488-66866, Clone: 1G2C6 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL488-66866

  1X10^6 Jurkat cells were stained with 0.2 ug CoraLite® Plus 488-conjugated Anti-Human CD81 (CL488-66866, Clone: 1G2C6) (green), or stained with 0.2 ug isotype control (black). Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66866 bindet in IF, FC CD81 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD81 fusion protein Ag27298
• Vollständiger Name: CD81 molecule
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC002978
• Gene symbol: CD81
• Gene ID (NCBI): 975
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).