CD81 Rekombinanter Antikörper

CD81 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

5A6

Kat-Nr. : CL647-65644

Synonyme

TAPA1, S5.7, CD81 molecule, CD81 antigen, 5A6

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  FC experiment of human peripheral blood leukocytes using CL647-65644

  1x10^6 human peripheral blood leukocytes were surface stained with 5ul CoraLite® Plus 647 Anti-Human CD81 (5A6) Mouse IgG2a RecAb (CL647-65644, Clone: 5A6) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL647-65644

  1x10^6 human peripheral blood leukocytes were surface stained with 5ul CoraLite® Plus 647 Anti-Human CD81 (5A6) Mouse IgG2a RecAb (CL647-65644, Clone: 5A6) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood leukocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65644 bindet in FC CD81 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Humane OCI-LY8-Zelllinie
• Vollständiger Name: CD81 molecule
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC002978
• Gene symbol: CD81
• Gene ID (NCBI): 975
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD81 (also known as TAPA1 or TSPAN28) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD81 is involved in signal transduction and cell adhesion in the immune system (PMID: 9597125). CD81 has also been identified as en essential receptor for HCV (hepatitis C virus) (PMID: 21428934).