CD82 Monoklonaler Antikörper

CD82 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E7A4

Kat-Nr. : 66803-1-PBS

Synonyme

CD82, KAI1, 1E7A4, CD 82, CD82,KAI1, IA4

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  WB analysis using 66803-1-Ig (same clone as 66803-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66803-1-Ig (CD82 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66803-1-Ig (CD82 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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  IF Staining of human ovary tumor using 66803-1-Ig (same clone as 66803-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human ovary tumor tissue using CD82 antibody (66803-1-Ig, Clone: 1E7A4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66803-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66803-1-PBS bindet in WB, IHC, IF-P, ELISA CD82 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD82 fusion protein Ag17513
• Vollständiger Name: CD82 molecule
• Berechnetes Molekulargewicht: 30 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC000726
• Gene symbol: CD82
• Gene ID (NCBI): 3732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD82 is a membrane glycoprotein and belongs to the tetraspanin superfamily, many of which are implicated in the regulation of cell motility, morphology, fusion, signaling, fertilization, and differentiation. CD82 was originally identified as a suppressor of metastasis located on human chromosome 11p11.2 in prostate carcinoma. The majority of evidence indicates that CD82 expression is downregulated or abolished in a variety of malignant tumors. CD82 is present at high levels in human monocyte and macrophage lineages and in various epithelial cells in the prostate, lung, pancreas and many other tissues. In epithelial cells, CD82 is implicated in diverse biological processes such as cell adhesion, migration, apoptosis and morphogenesis.